You would expect the wider-spaced data points to show greater movement. If you assume IID movements and defined variance, then you would expect the volatility-induced movement during an N-period interval to be SQRT[N] times greater. Thus, you could divide the more widely spaced data points by 1/SQRT(N) to normalize them for unequal spacing. This is a crude approximation as it also divides the drift or average daily gain by that factor. If you want avoid this, then subtract the estimated drift from the data and then normalize by 1/SQRT(N)If you really get into this concept you must start asking if the gap between data points over the week-end or holiday is the same as the gap between successive days (its not). This can lead to interesting ideas about market speed in which changes in volatility can be thought of as changes in a relative timebase. With that interpretation, high volume periods are often "faster" than low volume periods. That is, more market time passes per calendar time on intense days.